The process of freezing biological material like sperm, eggs, embryos, or tissues to sub-zero temperatures for later use is known as cryopreservation. They are thawed and used in a fertility cycle such as IUI, IVF, or ICSI. Cryopreservation allows cells to be frozen for a long period. It prevents cell death and DNA degradation. All biological activity is halted during the freezing process until the cells are thawed. Cryopreservation involves freezing sperm, eggs, embryos to -196°C/-321°F using liquid nitrogen. Cryoprotective chemicals are used in the lab to keep the cells safe during the process.